The Miniprep Song
(to the Tune Of Yesterday)
Minipreps...
Have an awful lot of spinning steps
But it seems there's not a better way
Of harvesting the DNA.
Thirty more...
Resuspending cells is quite a chore
My piptetting thumb is getting sore
Oh minipreps are such a bore!
Qia kits are great, 'cause I hate the phenol way
Still, next time you clone, clone by phone, it saves a day (hey hey hey)
Minipreps...
Know the worst thing about minipreps?
When you've finally gone through all the steps
It's time to do the maxipreps!
Oh dear God, not maxipreps!
Please, I'll do more
Minipreps ....
Minipreps...
Have an awful lot of spinning steps
But it seems there's not a better way
Of harvesting the DNA.
Thirty more...
Resuspending cells is quite a chore
My piptetting thumb is getting sore
Oh minipreps are such a bore!
Qia kits are great, 'cause I hate the phenol way
Still, next time you clone, clone by phone, it saves a day (hey hey hey)
Minipreps...
Know the worst thing about minipreps?
When you've finally gone through all the steps
It's time to do the maxipreps!
Oh dear God, not maxipreps!
Please, I'll do more
Minipreps ....
posted by Joolya at
12:22 PM
5 Comments:
You know we are accepting poetry for the Science Blogging Anthology....
awesome
I love doing minipreps. They keep you busy, they always work, you generally get useful information back. The qiagen kit is expensive, and overkill. I follow:
http://www.paralog.com/wiki/?PlasmidMiniprep
Very reliable. If you need it cleaned up more (sequencing?) you can add a touch of RNAse and put it over a spin column, but for restriction digests, they're fine.
When can we expect the digital version of you singing this song?!
Mini-preps, no need to stress
MacConnell Research lets you rest.
Purified right on the gel,
pipetted from the cassette well.
DNA so pure and true,
you won't thank me till I thank you.
Start a machine then walk away,
centrifuge some other day.
96 preps without a sweat,
seems just like a dream and yet,
it can be done, to find out how,
Call MacConnell Research now!
Sing to the tune of
"Wake up little lab girl, wake up"
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