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Designing reverse complementary strands plus restriction overhangs ... This is actually the most frustrating and difficult activity. Bloody genetic code.
Lab and non-lab-based adventures of a wandering cell biologist.
1 Comments:
yeah, but there are sticky ends - and i get hella confused about 5'-3' when it's all backwards-like!
(actually, for a while there i wasn't using the computer. that was mind numbing indeed.)
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